Student Work
Optimization of Procedures for Establishing Mouse Primary Cortical Cultures and siRNA Knockdowns for Studying Huntington's Disease Proteins
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open in viewerHuntington's disease (HD) is one of the most destructive progressive neurodegenerative disorders, and is caused by a genetic mutation in the HTT gene encoding Huntingtin. To assist Neil Aronin's ongoing HD research at UMass Medical Center, we developed a more efficient protocol for mouse cortical dissection and plating. In addition, we optimized a procedure for transducing cortical cells with lentiviruses containing various siRNAs and GFP, and assayed the percent knockdown by qRT-PCR. In the future, these procedures will help assess the role of previously identified protein partners of mutant Huntingtin that contribute to HD pathogenesis.
- This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
- Creator
- Contributeurs
- Publisher
- Identifier
- E-project-042511-124009
- Advisor
- Year
- 2011
- Sponsor
- Date created
- 2011-04-25
- Resource type
- Major
- Rights statement
Relations
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Contenu
Articles
La vignette | Titre | Visibilité | Embargo Release Date | actes |
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Tianna_and_Krista,_MQP_Final.pdf | Public | Télécharger |
Permanent link to this page: https://digital.wpi.edu/show/mw22v6925