The IMPAS family of proteases is not well characterized, but some members may be associated with neurodegenerative diseases, such as Alzheimer’s disease. In this project, potential IMPAS substrates and pathways were investigated in vitro by transfecting plasmids encoding various IMPAS proteins and potential substrates into HEK293 cells and monitoring the cellular levels of predicted substrate proteins by Western blots. The results show that ODZ4, Syntaxin 5A, and truncated ST14 (delta ST14) likely do not serve as substrates. Protease hIMP1 was validated to cleave HCV core protein, as in a previous report. A putative connection between IMPAS and the autophagy pathway was revealed.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Perreira, Jill Marie

Contributors

•  Dr. Evgeny Rogaev

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042612-124549

Advisor

• Adams, David S.

Year

• 2012

Sponsor

• University of Massachusetts Medical Center

Date created

• 2012-04-26

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright