Limb Regeneration in Horseshoe CrabsPublic
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Horseshoe crabs, like many invertebrates, are able to regenerate ablated appendages. It was hypothesized that ependymin and/or juvenile hormone would contribute to regeneration at ablation sites and could be demonstrated by immunohistochemistry. Ablated appendages and regenerated stumps were examined by light and electron microscopy. The post-ablation tissue retracts into the remaining limb where new shell is formed in between two layers of living tissue. JH immunoreactivity was found in blood cells in the regenerating stumps. Anti-EPN immunoreactivity was not detected.
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