-N-acetylgalactosaminidase is an enzyme that has several implicated applications in the biotechnology industry. in its productions from E. coli, there is an unknown inhibitor that is also co-produced. the goal of this project was to characterize and purify this inhibitor in hopes of the development of a more efficient means of purifying -N-acetylgalactosaminidase in the future. Many techniques for purification were used such as: Tangential Flow Filtration (TFF), Cation Exchange (CEX), Aqueous two-phase extraction( ATPE), Ammonium Sulfate precipitation, Hydrophobic Interaction Columns (HIC), as well as enzyme kinetic studies and inhibition kinetics.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Dacunha, Christopher Mark
• Eckley, Sean Timothy

Publisher

• Worcester Polytechnic Institute

Identifier

• 04D256M

Advisor

• DiIorio, Alexander A.

Year

• 2004

Date created

• 2004-01-01

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright