Student Work
Purification of an inhibitor of -N-acetylgalactosaminidase
Public-N-acetylgalactosaminidase is an enzyme that has several implicated applications in the biotechnology industry. in its productions from E. coli, there is an unknown inhibitor that is also co-produced. the goal of this project was to characterize and purify this inhibitor in hopes of the development of a more efficient means of purifying -N-acetylgalactosaminidase in the future. Many techniques for purification were used such as: Tangential Flow Filtration (TFF), Cation Exchange (CEX), Aqueous two-phase extraction( ATPE), Ammonium Sulfate precipitation, Hydrophobic Interaction Columns (HIC), as well as enzyme kinetic studies and inhibition kinetics.
- This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
- Creator
- Publisher
- Identifier
- 04D256M
- Advisor
- Year
- 2004
- Date created
- 2004-01-01
- Resource type
- Major
- Rights statement
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