Biophysical Characterization of Diacylglycerol Pyrophosphate Public
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Diacylglycerol pyrophosphate (DGPP), a phosphorylated form of phosphatidic acid (PA), gained attention due to its role as a signaling lipid. In plants, DGPP is virtually absent in non-stimulated cells but its concentration increases in response to various stress stimuli. It is yet unclear what its molecular function is and how it exerts its effects. To help characterize the functions of PA and DGPP, this study examined the lipids in pH 4 through 11 by infrared spectroscopy, and found that the pure DOPA multilamellar vesicles deprotonate to the PO32- species at lower pH than pure DGPP multilamellar vesicles, which is different than what has been found in previous studies. This study elucidated the ionization state and stretching frequencies of each lipid over the physiological pH range.
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