Using Proximity Labeling to Characterize the Protein Neighborhood of the Moss Exocyst Complex and Myosin XIPublic
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The moss Physcomitrium patens is an excellent model system to study tip growth in plants, in which exocytosis plays a key role. The exocyst complex and myosin XI are critical for exocytosis, but the interacting proteins of the moss exocyst and myosin XI are still unknown. To identify their associated proteins, moss lines were designed to express the proximity labeling enzyme TurboID, a promiscuous biotin ligase, fused to Sec6 and myosin XI. After biotin addition, the interacting proteins are targeted by biotinylation. Streptavidin beads were used to capture the biotinylated proteins, confirmed by Streptavidin Blot, and then sent to Mass Spectrometry for proteomic analysis.
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