The purpose of this project was to develop a novel in vitro culture method to enrich the natural killer (NK) cell population of an existing bioreactor system. A nonradioactive cell lysis protocol was also developed to assay NK functional activity. The data showed that this assay was a suitable substitute for the standard radioactive chromium-51 assay, and that functional NK cells were indeed produced in the culture system. The identity of the NK cells was further confirmed by FACS analysis.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Vanosdol, Margaret Ann

Publisher

• Worcester Polytechnic Institute

Identifier

• 99C002M

Advisor

• Adams, David S.

Year

• 1999

Sponsor

• t. Breeders Inc.

Date created

• 1999-01-01

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright