The objective of this Major Qualifying Project was to design and synthesize an artificial gene encoding a lobster vitellogenesis-inhibiting hormone (VIH) and then express that gene in host yeast cells. Based on the amino acid sequence of VIH, a set of oligonucleotides were designed and optimized for the codon bias of the yeast expression system, P. pastoris. The oligonucleotides were assembled into a single DNA molecule, cloned into a plasmid vector and sequenced, however, expression of the hormone is ongoing.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Hirsch, Erica Ann
• Kolpak, Adrianne Lynn

Publisher

• Worcester Polytechnic Institute

Identifier

• 03D071M

Advisor

• Bagshaw, Joseph C.

Year

• 2003

Date created

• 2003-01-01

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright