Recent developments in cancer research have shifted focus to personalized medicine to provide patients with individualized and effective cancer treatments. There is a lack of accurate ex vivo models that properly mimic tumor interactions and microenvironments. We have developed a protocol to create an in vitro, 3D, ring tumor model for use in cancer therapy testing. This model includes an agarose ring well, including a center post with a 2mm diameter at the base and a 2.5º inward taper, that was formed with a 3D printed punch that was designed using CAD. A mixed cell suspension of fibroblast cells (HMF-52) and GFP labeled breast cancer cells (MDA-MB-231) were incorporated into the ring models to represent tumor cells within healthy tissue. Fluorescence microscopy was utilized to observe cell distribution and behaviors within the rings after 4 days. Our design has shown evidence of microtumor integration within the ring, making the tissue ring setup a viable option for future tumor model development.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Raden, Elena
• Blake, Madeline J.
• Gonzales, Marissa M.
• Arnold, Kylie D.

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-050621-074931
• 22656

Keyword

• Tissue Ring
• Tumor Model
• Personalized Medicine
• Microtissue
• Treatment Testing

Advisor

• Page, Raymond L.

Year

• 2021

Date created

• 2021-05-06

Resource type

• Major Qualifying Project

Major

• Biomedical Engineering

Rights statement

• In Copyright

License

• All rights reserved