Engineered 3D Microtissues for Personalized Cancer TreatmentPublic
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Recent developments in cancer research have shifted focus to personalized medicine to provide patients with individualized and effective cancer treatments. There is a lack of accurate ex vivo models that properly mimic tumor interactions and microenvironments. We have developed a protocol to create an in vitro, 3D, ring tumor model for use in cancer therapy testing. This model includes an agarose ring well, including a center post with a 2mm diameter at the base and a 2.5º inward taper, that was formed with a 3D printed punch that was designed using CAD. A mixed cell suspension of fibroblast cells (HMF-52) and GFP labeled breast cancer cells (MDA-MB-231) were incorporated into the ring models to represent tumor cells within healthy tissue. Fluorescence microscopy was utilized to observe cell distribution and behaviors within the rings after 4 days. Our design has shown evidence of microtumor integration within the ring, making the tissue ring setup a viable option for future tumor model development.
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