It has been hypothesized that myosin and other cell components are located on vesicles to organize the actin network during polarized growth. Using confocal microscopy and fluctuation cross correlation analyses, I investigated the association of class II formins with vesicles and F-actin in Physcomitrella patens caulonemal cells. I found that formin2A co-localized with both VAMP, a vesicle marker, and Lifeact, an F-actin probe. Furthermore, formin2A fluctuated in phase with VAMP, but preceded Lifeact, in agreement with the hypothesis.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Pierce, Ellen Christine

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-043015-111047

Advisor

• Vidali, Luis

Year

• 2015

Date created

• 2015-04-30

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright