The dig-1 gene is proposed to be involved in axon guidance during C. elegans embryogenesis. Immunohistochemistry was used to investigate the expression pattern of several dig-1 alleles. Preliminary results showed staining during embryogenesis using an antibody generated against DIG-1 protein; however, controls suggest the antibody may not be specific. Various methods for immunohistochemistry were utilized to determine the most effective protocol and conditions for RT-PCR were optimized for future research of the dig-1 gene.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Carpenter, Laurie J.
• Giddings, Monica J.

Publisher

• Worcester Polytechnic Institute

Identifier

• 06D461M

Advisor

• Ryder, Elizabeth F.

Year

• 2006

Date created

• 2006-01-01

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright