Bioreactors produce cells for bioprocessing into biologics like vaccines and biopharmaceuticals. The cells required to inoculate a bioreactor are produced in a seed train, the steps taken to move from a frozen cell bank to a cell culture with a target density. The project used Vero cells, an adherent cell line. The goals of this project were: design a seed train for scaling up to the XDR-200 and evaluate microcarriers and single-use technology in the XDR-200. The group used tissue culture and spinner flasks to scale-up to the targeted cell density for the XDR-200. Cell counts and media analysis proved that the seed train was acceptable for scaling up using microcarriers and single-use technology. Future improvements include efficient trypsinization of cells and XDR-200 modifications.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Dalton, Nicholas James
• Larsson, Colin Anders
• Hill, Charles Anthony
• McKinzie, Zachary Alan
• Karam, Elie K.

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042717-111945

Advisor

• Reidinger, Zoe
• Rashid, Kamal

Year

• 2017

Sponsor

• General Electric Healthcare Life Sciences

Date created

• 2017-04-27

Resource type

• Major Qualifying Project

Major

• Biomedical Engineering

Rights statement

• In Copyright

Last modified

• 2021-02-02