Student Work

Seed Train Development for Vero Cells in the XDR-200

Public

Downloadable Content

open in viewer

Bioreactors produce cells for bioprocessing into biologics like vaccines and biopharmaceuticals. The cells required to inoculate a bioreactor are produced in a seed train, the steps taken to move from a frozen cell bank to a cell culture with a target density. The project used Vero cells, an adherent cell line. The goals of this project were: design a seed train for scaling up to the XDR-200 and evaluate microcarriers and single-use technology in the XDR-200. The group used tissue culture and spinner flasks to scale-up to the targeted cell density for the XDR-200. Cell counts and media analysis proved that the seed train was acceptable for scaling up using microcarriers and single-use technology. Future improvements include efficient trypsinization of cells and XDR-200 modifications.

  • This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
Last modified
  • 2021-02-02
Creator
Publisher
Identifier
  • E-project-042717-111945
Advisor
Year
  • 2017
Sponsor
Date created
  • 2017-04-27
Resource type
Major
Rights statement
License

Relations

In Collection:

Items

Items

Permanent link to this page: https://digital.wpi.edu/show/hd76s2831