Biochemical Characterization of the Interaction between Rab Proteins and Myosin XIa in the moss Physcomitrella patens Public
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In Physcomitrella patens, actin-mediated trafficking and myosin XIa are essential for polarized growth. In other model organisms, the association between myosin and its cargo is mediated by Rab-GTPases. We cloned and purified wild type and mutants of moss myosin XIa that fail to complement an RNAi myosin XIa loss-of-polarity phenotype. Through binding assays, we identified RabA21 as having potential myosin XIa binding activity. The disruption of this interaction may be responsible for the lack of rescue of the phenotype.
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