In Physcomitrella patens, actin-mediated trafficking and myosin XIa are essential for polarized growth. In other model organisms, the association between myosin and its cargo is mediated by Rab-GTPases. We cloned and purified wild type and mutants of moss myosin XIa that fail to complement an RNAi myosin XIa loss-of-polarity phenotype. Through binding assays, we identified RabA21 as having potential myosin XIa binding activity. The disruption of this interaction may be responsible for the lack of rescue of the phenotype.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Last modified

• 2023-01-20

Creator

• Garbarino, Jennifer Mary
• Kaptur, Sarah Elizabeth

Contributors

• Munson, Mary

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-043015-150051

Award

• Provost's MQP Award

Advisor

• Vidali, Luis
• Heilman, Destin

Year

• 2015

Sponsor

• UMass Memorial Medical Center

Date created

• 2015-04-30

Resource type

• Major Qualifying Project

Major

• Interdisciplinary

Rights statement

• In Copyright

License

• All rights reserved