Salmonella is a bacterial infection that results in an estimated 93.8 million cases and 155,000 deaths annually. Copper is an essential micronutrient that is toxic at high concentrations, and the ability of Salmonella to maintain copper homeostasis may be of importance to studying Salmonella’s pathogenicity. The GolS system plays a role in copper homeostasis in Salmonella, with its components, GolT and GolB, functioning as an inner membrane transporter and a cytoplasmic chaperone, respectively. In this study, we successfully generated golT and golB E. coli clones and studied the clone’s expression of the GolT and GolB proteins using arabinose to induce the pBAD vector. The clones were confirmed to be mutation-free through sequencing analysis. Our study also determined the optimal concentrations of arabinose for inducing protein expression using dot blot analysis. These findings provide a foundation for further investigations into the GolS system and its role in copper homeostasis in Salmonella.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Zhu, Leo

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-032223-100325
• 94101

Advisor

• Argüello, José M.

Year

• 2023

Date created

• 2023-03-22

Resource type

• Major Qualifying Project

Major

• Biochemistry

Source

• E-project-032223-100325

Rights statement

• In Copyright

Last modified

• 2023-04-13