This project examined the effects of cellobiose concentration, enzyme loading and other parameters in enzyme hydrolysis to yield maximum glucose. HPLC techniques were used to analyze the results of the experimental samples. It was observed that the cellobiose enzyme hydrolysis reaction process occurs very fast. The project determined the non-linear kinetics of the reaction, along with a suitable concentration range for continuing research. Recommendations were made outlining the steps to ensure future success for maximum glucose production via enzyme hydrolysis.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Paxton, Emma
• Hanscom, Hailey
• Mori, Kim

Subject

• Industry
• Energy
• Sustainability
• Project-Based Learning
• Environment

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042622-140553
• 63876

Keyword

• enzyme hydrolysis
• biofuel
• Glucose
• renewable energy
• Cellobiose

Advisor

• Timko, Michael T.

Year

• 2022

UN Sustainable Development Goals

• 7 - Affordable and Clean Energy
• 13 - Climate Action

Date created

• 2022-04-26

Resource type

• Major Qualifying Project

Major

• Chemical Engineering

Rights statement

• In Copyright