The utilization of Vero cells for vaccine production has contributed significantly to controlling viral diseases and improving the healthcare system in the US and around the world. In the United States, the adherent cell line Vero 76 has never been grown within a packed-bed bioreactor using Eppendorf’s BioFlo 320 system, however they are typically grown on microcarrier beads in a pitched-blade vessel. I compare a glass vessel to a single use vessel running in a semi-continuous mode of operation with daily glucose uptake and lactate production samples. The objective of this study was to determine if a packed-bed bioreactor can be seeded at a lower than typical density to ultimately decreases time, cost, and human risk during the seed train and scale-up process.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Guertin, Shannon Herlihy

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042816-154435

Advisor

• Rashid, Kamal
• Duffy, Joseph B.

Year

• 2016

Date created

• 2016-04-28

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright