Magnetic-activated cell sorting (MACS) was used to isolate and quantify antigen specific T cells. T cells specific to one vaccinia and three yellow fever peptides were isolated from two immune donors. Peptide specific cells were quantified by fluorescence-activated cell sorting analysis. Cytotoxic T lymphocyte (CTL) clones were generated from cultures obtained by the MACS assay through limiting dilutions. CTL assays on the bulk cultures and clones showed strong specific cytotoxic activity against target cells presenting their respective virus or peptide.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Danforth, Malinda J.
• Farak, Sonja J.

Publisher

• Worcester Polytechnic Institute

Identifier

• 00C024M

Advisor

• Rulfs, Jill

Year

• 2000

Date created

• 2000-01-01

Resource type

• Major Qualifying Project

Major

• Biochemistry
• Biology & Biotechnology

Rights statement

• In Copyright