Design of a Molding System to Recapitulate Skeletal Muscle Fiber
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open in viewerMany current systems for the development of myofibers involve the presence of a scaffold, which can be a physical barrier to cell proliferation and cause incongruities in the myofiber. We developed a molding system into which myogenic cells can be seeded to form a myofiber without the need for a scaffold. A "dog-bone" shaped molding system with a seeding channel and incorporated anchor posts was found to result in tissue formation one day after seeding. Characterization of C2C12 cells showed that differentiation occurs at Day 4 post-induction of differentiation and myosin staining of C2C12 cells confirmed maturation of resulting myofibers. Further studies should focus on improved manufacture of the mold to enhance the long-term stability of the myofiber.
- This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
- Creator
- Publisher
- Identifier
- E-project-042811-044811
- Advisor
- Year
- 2011
- Date created
- 2011-04-28
- Resource type
- Major
- Rights statement
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La vignette | Titre | Visibilité | Embargo Release Date | actes |
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Skeletal_Muscle_Regeneration_MQP_Final_Report.pdf | Public | Télécharger |
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