Many current systems for the development of myofibers involve the presence of a scaffold, which can be a physical barrier to cell proliferation and cause incongruities in the myofiber. We developed a molding system into which myogenic cells can be seeded to form a myofiber without the need for a scaffold. A "dog-bone" shaped molding system with a seeding channel and incorporated anchor posts was found to result in tissue formation one day after seeding. Characterization of C2C12 cells showed that differentiation occurs at Day 4 post-induction of differentiation and myosin staining of C2C12 cells confirmed maturation of resulting myofibers. Further studies should focus on improved manufacture of the mold to enhance the long-term stability of the myofiber.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Kalluri, Deepti
• Donado, Carlos Andres
• Mathieson, Derek L.

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042811-044811

Advisor

• Page, Raymond L.
• Adams, David S.

Year

• 2011

Date created

• 2011-04-28

Resource type

• Major Qualifying Project

Major

• Biomedical Engineering
• Interdisciplinary

Rights statement

• In Copyright