The exocyst complex plays roles in membrane trafficking and fusion during exocytosis. Current Cryo-EM models do not provide clear structural information on the complex or its CorEx bundles. CorEx bundles are involved in connecting the two exocyst modules, though the mechanism is poorly understood. We attempted to obtain a crystallized structure of a CorEx bundle by two methods: building the complex using a polycistronic plasmid system and digesting the complex by limited proteolysis.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Pearce, Camille S.

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-051820-131929

Advisor

• Farny, Natalie
• Munson, Mary

Year

• 2020

Sponsor

• UMass Medical School

Date created

• 2020-05-18

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright