Assessing the Efficacy of Known Procambarus clarkii Primers to Amplify Microsatellite Loci in Orconectes limosus and O. quinebaugensis Public
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The goal of this project was to assess the efficacy of microsatellite primers in three crayfish species present in Massachusetts. We began by investigating twenty primer pairs previously developed for Procambarus clarkii. After selecting three loci for further study, we explored the applicability of those three primer pairs in two additional species of crayfish, Orconectes limosus and O. quinebaugensis. Resulting products from P. clarkii were sent to Cornell University for fragment analysis, but the analysis proved inconclusive.
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