Effect of Promensil on T47D Breast Cancer Cells with respect to Estrogen Receptors Alpha and Beta

Jankowski, Matthew R.; Sullivan, Shea E.; Zarate, Christine M.

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Effect of Promensil on T47D Breast Cancer Cells with respect to Estrogen Receptors Alpha and Beta Public

Promensil, an OTC treatment for menopausal symptoms, is known to reduce breast cancer cell number in vitro. Studies have also shown that phytoestrogens found in Promensil bind to estrogen receptors (ERs). This study investigates whether the reduction in cell number is due to phytoestrogen binding to ER. Using T47D-KBluc cells, a luciferase assay was used to see if phytoestrogens in Promensil bind to ERs. The effect of receptor antagonists on cell number was also investigated. Interestingly, at doses that decrease cell numbers, phytoestrogens did not appear to bind to ERs. Cell numbers also decreased when ER binding was inhibited. These data suggest that changes in cell number are not mediated through ERs. Further study may show whether proliferation rates decreased or apoptosis increased.

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