Regulation of Gene Expression by 5' UTRs in Mycobacterium smegmatis Public
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Tuberculosis, caused by Mycobacterium tuberculosis, is a highly infectious disease. TB is unique in that it can survive stress in the human body, including hypoxia. A possible survival mechanism is the influence of 5' UTRs on gene regulation. To test this, a series of constructs were built to include a strong promoter and a fluorescent reporter gene. One construct introduced a degradation tag; another removed the MOP associated 5' UTR. Constructs were assessed by fluorescence microscopy and colony color to evaluate mCherry expression. Findings revealed that the degradation tag was too efficient and the UTR was unnecessary. The final construct contains the MOP promoter and the mCherry reporter gene and can be used in future studies to test the impact of various 5' UTRs on gene expression.
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