Development of a Novel scRNAseq Pipeline and Analysis of Influenza-Infected Mouse Lung Cells

Raven, Emmaline

Student Work

Development of a Novel scRNAseq Pipeline and Analysis of Influenza-Infected Mouse Lung Cells

Public Deposited

This project developed a comprehensive pipeline for scRNAseq analysis of a large dataset, including statistically sound quality control measures, differential gene expression analysis, and cell type annotation. All of these analyses were performed using open-source packages developed for R scripting. The pipeline was used to analyze a dataset from an experiment on the effects of paternal stress on the disease tolerance of offspring through epigenetic inheritance. 22 cell type clusters were identified in populations of mice with and without paternal exposure to stress (Influenza A Virus infection); clusters were annotated by analyzing marker gene expression. Further analysis of the results of this project may help to determine potential mechanisms of disease tolerance.

This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator