EXAMINING THE ENCEPHALO-ANTIGENIC AND HLA-DR BINDING CAPACITIES OF VARIOUS MYELIN PEPTIDES
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open in viewerActivated CD4+ T-cells targeting specific auto-antigenic peptides of myelin proteins mediate the pathogenesis in multiple sclerosis patients and EAE models. The disease mechanisms that cause MS remain unknown, but susceptibility maps to specific HLA-DR beta- 1 alleles (especially 04.01, and 15.01). To investigate the genetic predisposition of MS recombinant HLA-DR beta-1 complexes (01.01, 04.01, and 15.01) were constructed containing different peptides derived from sheath proteins: PLP, MBP, and MOG. The gene constructs were transfected into SF9 cells to evaluate surface expression as it relates to the auto-antigenic activity of several pMHC combinations. Individual myelin peptides were also assayed for the ability to induce EAE in transgenic HLA-DR beta-1 04.01 and 15.01 mice.
- This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
- Creator
- Publisher
- Identifier
- E-project-043015-134808
- Advisor
- Year
- 2015
- Sponsor
- Date created
- 2015-04-30
- Ubicación
- New York
- Resource type
- Major
- Rights statement
Las relaciones
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Elementos
Elementos
Miniatura | Título | Visibilidad | Embargo Release Date | Acciones |
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4-30-15_Luisanna_MQP_Final.pdf | Público | Descargar |
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