Research at WPI is attempting to create reliable microbial biosensors which could detect chemicals underground in soil. To implement this system, the expression levels of a chassis organism, Pseudomonas putida, were analyzed using previously published P. putida RNA-seq data. Employing KBase, a new cloud-based bioinformatics tool, narratives were created to produce gene expression matrices (FPKM values) for evaluation of constitutive promoters and upregulated genes. Our results identified infC, atpI, and ccO-I promoters as potential promoters for constructing a robust biosensor.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Harding, Kyle R.

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-050621-082631
• 22671

Advisor

• Young, Eric Mosher
• Farny, Natalie

Year

• 2021

Date created

• 2021-05-06

Resource type

• Major Qualifying Project

Major

• Bioinformatics & Computational Biology

Rights statement

• In Copyright

Last modified

• 2021-08-29