Transcriptomics Analysis of Pseudomonas Putida for Implementation of Soil Microbial BiosensorPublic
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Research at WPI is attempting to create reliable microbial biosensors which could detect chemicals underground in soil. To implement this system, the expression levels of a chassis organism, Pseudomonas putida, were analyzed using previously published P. putida RNA-seq data. Employing KBase, a new cloud-based bioinformatics tool, narratives were created to produce gene expression matrices (FPKM values) for evaluation of constitutive promoters and upregulated genes. Our results identified infC, atpI, and ccO-I promoters as potential promoters for constructing a robust biosensor.
- This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
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