The Sec 61 complex in eukaryotes is a passive pore in the endoplasmic reticulum (ER) membrane that permits a secretory protein to enter the ER membrane or lumen. To facilitate understanding of channel opening and closing, and especially to help determine the location of the plug domain relative to key cysteine residues, several yeast Sec 61 mutants were constructed by site-directed mutagenesis of cysteine residues at positions C121, C150, and C373. The mutants expressed Sec 61 and showed growth defect phenotypes.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Li, Jeffrey

Contributori

• Gilmore, Reid

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042711-104235

Advisor

• Adams, David S.

Year

• 2011

Sponsor

• UMASS Medical Center

Date created

• 2011-04-27

luogo

• Worcester

Resource type

• Major Qualifying Project

Major

• Biochemistry

Rights statement

• In Copyright

Ultima modifica

• 2023-10-09