Student Work

CONSTRUCTION OF MUTANTS FOR A CYSTEINE ACCESSIBILITY ASSAY FOR THE YEAST SEC61 COMPLEX

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The Sec 61 complex in eukaryotes is a passive pore in the endoplasmic reticulum (ER) membrane that permits a secretory protein to enter the ER membrane or lumen. To facilitate understanding of channel opening and closing, and especially to help determine the location of the plug domain relative to key cysteine residues, several yeast Sec 61 mutants were constructed by site-directed mutagenesis of cysteine residues at positions C121, C150, and C373. The mutants expressed Sec 61 and showed growth defect phenotypes.

  • This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
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Identifier
  • E-project-042711-104235
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Year
  • 2011
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Date created
  • 2011-04-27
Location
  • Worcester
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Major
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Last modified
  • 2023-10-09

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Permanent link to this page: https://digital.wpi.edu/show/d791sh906