DIG-1 appears to be an adhesion protein involved in neuron guidance during C. elegans nervous system development. This MQP attempted to show recognition of, as well as specificity to DIG-1 by anti-con3, a previously raised antibody to the C-terminus. Various methods of cutting the protein were employed and analysis was performed using polyacrylamide gel electrophoresis. Fluorescence was also used to look at in vivo localization of DIG-1. Results suggest that anti-con3 does not specifically recognize DIG-1 from a protein homogenate.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Dasso, Robert Anthony
• Rosinha, Elizabeth Merry

Publisher

• Worcester Polytechnic Institute

Identifier

• 05D514M

Advisor

• Ryder, Elizabeth F.

Year

• 2005

Date created

• 2005-01-01

Resource type

• Major Qualifying Project

Major

• Biochemistry
• Biology & Biotechnology

Rights statement

• In Copyright