Latent TB infections are a result of the diseases' ability to evade host immune defenses and remain in a quiescent asymptomatic state. Clonal expansion of T-cells within granulomas of individuals with active and latent TB provides an opportunity to determine the antigens capable of promoting an adaptive immune response within a human subject. Using sequence information from T-cells of patients with Latent Tuberculosis, T cell receptor (TCR) variable region inserts were designed. The objective of this work is to utilize Golden Gate cloning of the compiled TCR sequences to create a repertoire of specific TCRs for eventual expression using retroviral vectors.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Studley, Maxwell Conrad

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042519-065548

Advisor

• Rulfs, Jill

Year

• 2019

Sponsor

• UMMS - Department of Microbiology and Physiologica

Date created

• 2019-04-25

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright