Mapping Conditional Loss of Growth Mutations in Physcomitrella patens

Pervere, Leah Marie

Student Work

Mapping Conditional Loss of Growth Mutations in Physcomitrella patens

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To identify genes important for growth, we generated conditional mutants that experience loss of growth at high temperature (CLoG mutants). We outcrossed one of these mutants, CLoG4, to a polymorphic strain and sequenced the whole genome of a pool of segregants that maintained temperature sensitivity to map the mutation causing the phenotype. I also performed a rescue experiment to confirm the identity of the phenotype-causing mutation of a second strain, CLoG1, which had already been mapped.

This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

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