DESIGN OF VIRAL VECTORS FOR EXPRESSING INFLUENZA PROTEINSPublic
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Influenza is a major cause of human illness and death, and as such requires vast attention.Current vaccine research shows that human sera have influenza virus-specific antibodies which mediate the complement-dependent lysis(CDL) of viral infected cells and these antibodies may be more cross-reactive than typical neutralizing antibodies.This project focused on the development of a protein expression system that would allow human A549 cells to express influenza proteins to be used in analyzing the specificity of CDL antibodies.The results show that a lentiviral expression system is not the best method of expression; only H3 protein expression was confirmed by immunoblotting. Several influenza gene constructs were then successfully constructed for future vaccinia virus expression experiments.
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