Mechanism of Near-Infrared Fluorescent Signal Generation in Proteolysis-Sensitive Macromolecular Imaging Probes
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open in viewerIn vivo optical imaging of proteolysis in inflammatory and tumor microenvironments can be monitored through excitable fluorophore-labeled probes using a graft copolymer of poly(ethylene glycol) and poly-L-lysine as a carrier molecule. In this project, both in vitro and cell culture experiments were performed to investigate the major mechanisms of PGC-fluorophore probe cleavage via stromal cell proteases. It was observed that trypsin-mediated cleavage of PGC-fluorophore probes results in distinct fragment sizes, a 2-fold increase in fluorescence intensity, and a rapid 1.5-fold increase in fluorescence lifetime. Finally, it was observed that human squamous carcinoma cells uptake probes faster than normal human dermal fibroblasts with a greater fluorescence intensity over time.
- This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
- Creator
- Publisher
- Identifier
- E-project-042413-193311
- Advisor
- Year
- 2013
- Sponsor
- Date created
- 2013-04-24
- Resource type
- Major
- Rights statement
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Jessica_Lopez_MQP_Final_Report.pdf | Public | Download |
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