Early embryogenesis and cell fate specification of Caenorhabditis elegans is mainly driven by post-transcriptional regulation, involving RNA binding-proteins that bind to the 3' UTRs of maternal mRNAs. Regulation of this binding activity is crucial to the maturation of the zygote since this process ensures proper formation of the body plan. The goal of this project is to understand the regulation of different 3’ UTRs by different RNA binding proteins. To study this regulation, it is necessary to study the expression patterns driven by 3’UTRs using transgenic strains. To achieve this, we used a library MosSCI approach to generate multiple reporter strains in a faster way.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Donohue, Alyssa E.

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-050114-111540

Advisor

• Heilman, Destin

Year

• 2014

Sponsor

• University of Massachusetts Medical School

Date created

• 2014-05-01

Resource type

• Major Qualifying Project

Major

• Biochemistry

Rights statement

• In Copyright