In this study, the binding of IQGAP1 to Akt1 and EGFR in the HeLa cell line was investigated using Fluorescence Lifetime Imaging Microscopy. It was confirmed that Akt1 and EGFR do interact in the presence of IQGAP1. Additionally, an increased interaction between PIP3 and EGFR was observed upon stimulation with EGF. This interaction was found to be mediated through IQGAP1 scaffolding, which suggests that the scaffold plays a key role in modulating the PI3K/Akt/mTOR signaling pathway's response to pro-migratory stimuli such as EGF.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Lindberg, Samantha Kathryn

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042219-145651

Advisor

• Gericke, Arne

Year

• 2019

Date created

• 2019-04-22

Resource type

• Major Qualifying Project

Major

• Biochemistry

Rights statement

• In Copyright