MEX-3 BINDS TO A CONSERVED FRAGMENT OF THE NOS-2 3'- UNTRANSLATED REGION Public
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Normal development during early embryogenesis in Caenorhabditis elegans is contingent upon both the temporal and spatial expression of several mRNAs. In the early embryo no transcription takes place, so the asymmetric expression of cell-fate determinants is reliant on post-transcriptional regulators. MEX-3 is an RNA-binding protein containing two KH-domains that is expressed in the proximal gonad arm and the anterior blastomeres and P granules in the early embryo. In this MQP, EMSAs and titration fluorescence polarization were employed to test the binding specificity of MEX-3 to the 3' UTR of nos-2 mRNA. This MQP provides in vitro evidence that MEX-3 binds to domain subC of the 3'-UTR of nos-2, and establishes a basis for subsequent in vivo experiments.
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