The internal transcribed spacers (ITS1 and ITS2) of the nuclear ribosomal genes were amplified through PCR for 15 different populations of Artemia brine shrimp. Each PCR product was digested with 4 different restriction enzymes to produce restriction fragment patterns which were scored using the Restdist and Dollop programs from PHYLIP. Results indicate that the marker is highly conserved even among parthenogenetic populations while also suggesting a possible subdivision of the parthenogenetic clade based on geographical locations.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Perez-Torres, Carlos Jose

Publisher

• Worcester Polytechnic Institute

Identifier

• 07D077M

Advisor

• Bagshaw, Joseph C.

Year

• 2007

Date created

• 2007-01-01

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright