Reactive oxygen species (ROS) are reduced forms of oxygen created as byproducts of cellular metabolic processes. ROS cause oxidative damage to many organic molecules within the cell, especially to DNA. OXR2 provides cells with protection against this damage, but its biochemical and functional properties have not yet been studied in depth. The purpose of this project was to create and purify a His-tagged version of human OXR2 and to induce a FLAG-tagged OXR2 in E. coli. These tagged proteins will be used in studies of this protein and its mechanism.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Tashjian, Tommy F.

Contributori

• Volkert, Michael

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042409-193454

Advisor

• Adams, David S.

Year

• 2009

Sponsor

• University of Massachusetts Medical Center

Date created

• 2009-04-24

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright