Development of a Two-Hybrid System for Rapidly Detecting Flaviviral Infections
PublicStudies have shown that an innate immune response occurs during viral infection of mammalian cells when two protein components, TLR-3 and TRIF, bind together after recognition of foreign RNA inside a cell. The TLR-3/TRIF interaction was used to design a fluorescence-based two-hybrid system for rapidly detecting Flaviviral infections. A plasmid encoding TRIF was engineered to express half of a Venus (YFP) reporter, while TA cloning was used to confirm the clonability of the TLR-3 gene. Future experiments include cloning the TLR-3 gene into the Venus vector, and transfecting the fusion proteins into Vero cells. Upon addition of a sample containing viral RNA to this cell-line, the modified TLR-3 and TRIF proteins should dimerize, allowing the YFP to assemble, causing the cell to fluoresce.
- This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
- Creator
- Contributeurs
- Publisher
- Identifier
- E-project-042810-164831
- Advisor
- Year
- 2010
- Sponsor
- Date created
- 2010-04-28
- Resource type
- Major
- Rights statement
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Erin_Burns_and_Maura_Craig,_MQP_Final[1].pdf | Public | Télécharger |
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