The Gαq-PLCβ pathway is triggered when a neurotransmitter, hormone, or other signalling molecule binds to a G-protein coupled receptor located on the surface of the cell membrane. This results in a series of steps that lead to increased levels of cellular calcium ions. In this project, fluorescent sensor dyes and intrinsic fluorescence of NADH/NAD+ were used to show that increased levels of intracellular calcium ions after carbachol stimulation occur in parallel with increased mitochondrial activity and oxidative stress in the mitochondria.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Vail, Emily S.

Subject

• Health

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-050621-132132
• 22971

Keyword

• mitochondria
• stress
• calcium
• FLIM

Advisor

• Scarlata, Suzanne Frances

Year

• 2021

Date created

• 2021-05-06

Resource type

• Major Qualifying Project

Major

• Biochemistry

Rights statement

• In Copyright

Last modified

• 2021-08-29