CBF Regulation on RAG Genes Via Erag Enhancer
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open in viewerThis project explores the function of Core Binding Factor (CBF) in B-cell development, and its regulation on the recombination activating genes (RAG) that encode the B-cell antibody gene recombinase via Erag enhancer. A GFP reporter construct containing Erag and the RAG2 promoter was transfected into pro-B cells, and was mutated to block CBF binding. Leukemia cells and transfected pro-B cells were treated with CBF inhibitor to measure the effects of disrupted CBF function. The transcript levels of B-cell specific proteins of inhibitor-treated cells were measured by qPCR. The data indicate cell viability and key B cell protein expressions were decreased, but reporter activity was not decreased after inhibitor treatment. The mutant was obtained but was not yet transfected.
- This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
- Creator
- 贡献者
- Publisher
- Identifier
- E-project-042911-104646
- Advisor
- Year
- 2011
- Sponsor
- Date created
- 2011-04-29
- Resource type
- Major
- Rights statement
- 最新修改
- 2023-10-09
关系
- 属于 Collection:
项目
Permanent link to this page: https://digital.wpi.edu/show/8336h3765