CBF Regulation on RAG Genes Via Erag Enhancer

Huang, Zhao

Student Work

CBF Regulation on RAG Genes Via Erag Enhancer

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This project explores the function of Core Binding Factor (CBF) in B-cell development, and its regulation on the recombination activating genes (RAG) that encode the B-cell antibody gene recombinase via Erag enhancer. A GFP reporter construct containing Erag and the RAG2 promoter was transfected into pro-B cells, and was mutated to block CBF binding. Leukemia cells and transfected pro-B cells were treated with CBF inhibitor to measure the effects of disrupted CBF function. The transcript levels of B-cell specific proteins of inhibitor-treated cells were measured by qPCR. The data indicate cell viability and key B cell protein expressions were decreased, but reporter activity was not decreased after inhibitor treatment. The mutant was obtained but was not yet transfected.

This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

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