Interactions between DNA repair proteins Rad51 and XRCC3 were explored to investigate their roles in homologous recombination with respect to breast cancer. Recombinant plasmids and mammalian cell lines containing various XRCC3 mutants were constructed for experimentation with yeast hybrid and MMC sensitivity assays to test XRCC3 interaction with Rad51 or Rad51C in repair. Our results confirmed physical interactions between XRCC3 and Rad 51 or Rad51C, respectively. Mutations within or near the ATP-binding domain of XRCC3 almost always completely disrupted interaction with Rad51.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Banach, Julie Jacqueline
• Roy, Christian Knauf

Publisher

• Worcester Polytechnic Institute

Identifier

• 04D015M

Advisor

• Adams, David S.

Year

• 2004

Date created

• 2004-01-01

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright