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Mapping the domain structure of Ribonuclease E in Mycobacterium smegmatis

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Mycobacterium tuberculosis is a bacterium that causes tuberculosis. Regulation of gene expression and mRNA degradation is important for the ability of the bacteria to survive the stressors of infection. A multiprotein complex called the degradosome is important for mRNA degradation in better-studied bacteria such as E. coli, where Ribonuclease E (RNase E) cleaves mRNA into fragments while using a scaffold domain to interact with other proteins involved in mRNA degradation. We used M. smegmatis, a non-pathogen relative of M. tuberculosis, as a model to define the putative scaffold domains of mycobacterial RNase E. Our results suggest that RNase E has two scaffold domains flanking a putative catalytic domain 493 amino acids in length.

  • This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
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  • E-project-042519-163145
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  • 2019
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  • 2019-04-25
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Permanent link to this page: https://digital.wpi.edu/show/6t053j702