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Investigating a Potential Sly1/Sed5 Binding Site Public

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Eukaryotic vesicle targeting and fusion are conserved processes that involve SNARE and SM proteins. The SM protein Sly1 and its cognate SNARE Sed5 function in trafficking between the ER and the Golgi. It is known that Sly1 binds to the N-terminal peptide of Sed5. To investigate an alternative binding, a truncated Sed5 construct, Sed5 (23-324), was designed. This construct was cloned, expressed in E. coli and S. cerevisiae, and purified. Binding studies were conducted and the results indicated that the truncated Sed5 (23-324) did not bind to Sly1, suggesting that the N-terminal binding site may be the only binding domain.

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  • E-project-042412-200404
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  • 2012
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  • 2012-04-24
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Permanent link to this page: https://digital.wpi.edu/show/6h440v061