In Multiple Sclerosis patients, previous experiments have determined the structure, potential function, and regulation of the oxidative damage resistance protein nuclear receptor coactivator-7 alternate start (NCOA7-AS) through induction with Interferon-Beta (IFN-Beta). However, the subcellular localization of NCOA7-AS remains unknown. In this project, the localization will be determined by fusing GFP to NCOA7-AS, expressing the fusion protein in HT1080 and HeLa cells, and using fluorescence microscopy. Cells will also be treated with IFN-Beta to see if this alters NCOA7-AS localization. The GFP microscopy data indicate that NCOA7-AS localization is diffuse in cells with no clear subcellular localization, and that addition of IFN-Beta does not alter the localization.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Camire, Andrew Cole

Contributors

• Volkert, Michael

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042213-140454

Advisor

• Adams, David S.

Year

• 2013

Sponsor

• University of Massachusetts Medical School

Date created

• 2013-04-22

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright