The microalgae Ettlia oleoabundans produces up to 80% of its dry weight in triacylglycerols and is a prime candidate for biodiesel production. This study explores methods for transformation of E. oleoabundans by electroporation. Both the antibiotics hygromycin and spectinomycin were effective for transformant selection. First apparent successful production of E. oleoabundans protoplasts was achieved. Because this species produces nearly wall-less zoospores during its life cycle, synchronization was attempted using light/dark cycling and nitrate starvation to increase concentrations of zoospores to improve transformation.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Carter, Brant Christian

Colaboradores

• Arsenault, Patrick

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042810-154810

Advisor

• Weathers, Pamela

Year

• 2010

Date created

• 2010-04-28

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright