Development of a System to Culture and Evaluate Leaf-Derived Vascular Scaffolds (LeaVS) for Skin Regeneration

Grandhi, Nishan

Student Work

Development of a System to Culture and Evaluate Leaf-Derived Vascular Scaffolds (LeaVS) for Skin Regeneration

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There are many challenges with the use of engineered skin substitutes including their high cost, fragility, and long culture time. However, the one feature that is missing from many of these substitutes is that there is limited vasculature. George Pins’ lab is currently researching the use of decellularized spinach leaves to serve as skin substitutes with its already built-in vascular network. The device currently used for culturing skin cells on the leaves, although effective, has flaws such as its ability to perfuse the scaffold vasculature and to be assembled quickly. The goal of this project is to design and develop a sterile housing device which allows for imaging, user friendliness, and perfusion. Testing will show proof of concept. Results suggest our device to be a viable tool for user friendliness and bilayered cell seeding during perfusion.

This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

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