When treated with interferon-beta-1b, gene NCOA7 expresses a new variant that begins from an alternative start site NCAO7-AS with strong antioxidant properties. Segments of three different lengths of the upstream region of the gene were inserted into phRLnull. After testing and confirming their structure, the plasmids were transfected into mammalian cells. A luciferase assay was performed to determine which segments showed an increase in activity when treated with interferon. While each ligated plasmid acted as a strong promoter for Renilla luciferase, no significant difference in signal due to INF-beta- 1b treatment was observed.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Koloski, Tyler James

Contributori

• Volkert, Michael

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042513-151929

Advisor

• Adams, David S.

Year

• 2013

Sponsor

• UMass Medical School

Date created

• 2013-04-25

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright

Ultima modifica

• 2023-09-28