Over-expression of Vascular Endothelial Growth Factor-A (VEGF-A) can lead to a variety of human diseases, including different forms of cancer, psoriasis, and arthritis. The goal of this project was to develop a cell culture-based assay using human umbilical vein endothelial cells (HUVECs) to identify potential inhibitors of the VEGF-Receptor-2 (VEGFR2) and potential blockers of angiogenesis. After testing a variety of assays, the Hoechst nuclear stain was found to be convenient assays for quantifying cell proliferation. A screen of a chemical compound library using this Hoechst assay identified BSB660 as a novel inhibitor of VEGFR2. In the future, other chemical libraries will be tested using a TDA in vitro assay under development by other Blue Sky employees.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Beovich, Kerrin Marie

Contributeurs

• Navaroli, Deanna

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042413-171447

Advisor

• Adams, David S.

Year

• 2013

Sponsor

• Blue Sky Biotech, Inc.

Date created

• 2013-04-24

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright